Microbial Transformation of Steroids Essay Example for Free

Microbial Transformation of Steroids Essay Abstract Hydroxylations are possibly the most widespread type of steroid bioconversion. Hydroxylations can be used to buildintermediates for further chemical synthesis to provide the steroid molecule with the adequate structure for therapeutic applications. Microorganisms able to hydroxylate steroids in positions C1 to C21 and in position C26 have been reported. 11 ÃŽ ± hydroxylation is regarded as essential for anti-inflammatory action. Present study was promoted to extend the investigations to evaluate potentiality of this local oil contaminated soil isolates for transformation of progesterone. Enrichment is carried out in the inorganic medium providing progesterone as an only carbon source. Metabolites are identified by GC/MS and isolates by 16 s rRNA. 11 ÃŽ ± hydroxyprogesterone is the metabolite identified having 60 percent of yield. and Bacillus circulans Tex 01-S3c has been found most efficient strain. Key words: Hydroxylation of Progesterone, oil contaminated, Bacillus circulans Tex 01-S3c, INTRODUCTION Application of molecular biology techniques and genetic engineering of microorganisms for their improvement as steroid transforming agents are the areas of drug development. Site selection oxygenation, particularly hydroxylation of exogenous steroids is frequent in bacteria genera Bacillus. Several microbial bioconversion of steroids and sterols have been reported, focusing mainly on steroid hydroxylations, ∆1-dehydrogenation and sterol side-chain cleavage. These biotransformations, mostly associated to chemical synthesis steps, have provided adequate tools for the large scale production of natural or modified steroid analogues. The complex structure of the steroid molecule requires complicated, multi-step schemes for the chemical synthesis of steroid compounds. Microbial steroid conversions are performed in mild temperature and pressure conditions and can provide an efficient alternative to chemical synthesis. Screening and isolation of active microbial strains for steroid bioconversion is presently an important part of the research and development effort in the steroid drug industry. Oil contaminated soil is a rich source of variety of bacteria capable of biotransformations and degradations of complex chemicals. MATERIAL AND METHODS 1 gm of oil contaminated soil from different local places was added in the sterile 9 ml saline and 1 ml from this is added in the sterile progesterone liquid enrichment medium (KH2PO4, Na2HPO4. 12 H2O, (NH4)2SO4, MgSO4. 7 H2O, CaCl2. 6H2O, FeSO4. 7 H2O, pH 7) providing progesterone as a only carbon source. As progesterone is not soluble in water, it was dissolved in acetone (50 mg / ml). The flasks are incubated at 300 C for 7 days. Regular microscopic observation and turbidity checking carried out. By serial dilution techniques 0.1 ml was spread on the nutrient agar plates and well isolated colonies are studied for biotransformation potential. The medium of composition (g/liter): glucose, 40.0; peptone, 1.0; KH2PO4 0.74; MgSO4. 7H2O, 1.0; Yeast extract, 1.0 and asparagine, 0.7, pH was adjusted to 7 used for transformation. The cultivation was performed in 250-ml Erlenmeyer flasks each containing 50 ml medium. The flasks were sterilized and inoculated with 2 ml inoculum of 24 hr cultures of the pure organism. The culture flasks were incubated on incubator shaker at 34Â ° C for 48 hr. Thereafter, 50 mg of progesterone, dissolved in 1 ml acetone, was added to each flask and fermentation was continued for another 72 hr. At the end of fermentation, metabolites are analyzed by GC/MS. The efficient bacterium identified by 16S rRNA. Standardization of parameters like temperature, pH, incubation period, carried out spectrophotometrically. RESULTS AND DISCUSSION 11 ÃŽ ± hydroxyprogesterone is the metabolite identified having 60 percent of yield. Bacillus circulans Tex 01-S3c is the efficient bacterium identified. Temperature 340 C, pH 6.5, incubation period 60 hours are the optimum parameters for maximum yield. REFRENCES 1. Al-Awadi, S., Afzal, M. and Oommen, S. (2003) Studies on Bacillus stearothermophilus. Part III. Transformation of testosterone. Applied Microbiology and Biotechnology 62 , pp. 48-52. 2. Garai S, Banerjee S, Mahato SB (1995). Selective 1-dehydrogenation of progesterone by Aspergillus fumigatus. J Chem Res (Suppl): 408-409. 3. Fernandes, P., Cruz, A., Angelova, B., Pinheiro, H. and Cabral, J. (2003) Microbial conversion of steroid compounds: recent developments. Enzyme and Microbial Technology 32 , pp. 688-705 4. Mahato, S.B. and S. Garai, 1997. Advances in microbial steroid biotransformation. Steroids, 62:332-345. 5. Sameera Al-Awadi, M.A. Fzal and S. Oommen, 2001. Studies on Bacillus stearithermophilus part Transformation of progesterone to a new metabolites 9,10-seco-4-pregnene-3,9,20-trione, Journal of steroid biochemistry and Molecular biology; 78: 493-498. 6. Smith, KF and Kirk, DN (1989) Microbial transformation of steroids-II. Transformations of progesterone, testosterone an d androstenedione by Phycomyces blakesleeanus. Journal of Steroid Biochemistry 32 , pp. 445-451

Possessing The Secret Of Joy: Four Men To Find A Cure :: essays research papers

Four Men to Find a Cure The four main men in Possessing the Secret of Joy have roles that contradict a stereotypical male; they are the cure to Tashi's happiness. Alice Walker gives Adam, Mzee, Pierre, and Benny roles that show a softer side to men. These four men are very different from each other but they do have some resemblance of each other. These men who were all very devotedly attached to Tashi took care of her and never gave up on her. Instead of deceiving and being indolent, these four men were very likeable. The men tend to symbolize and represent different aspects of Tashi's life. Tashi's husband Adam symbolized love. He was the only lover of Tashi that we read about. He cared for her and watched over her even when he did not have control over her. Adam may not have been loyal to Evelyn, but he loved her and took care of her. He knew the real Tashi that many people never saw. He was loyal to Tashi, but after the circumcision he would barely ever see her. He was more of a great friend than a good husband was. He lets her know that he loves her several times in the book. At their marriage he cuts the same lines in his face as Tashi had to represent the Olinkans. He goes with her to Mzee's house in Switzerland while she is cured. He also dreams about her and her livelihood, like when she used to say, "But what is it?" when she was happy. Adam, Olivia's brother, was daring at heart. He and Tashi used to go the fields to have sex that was considered very taboo. His mentors were Lisette and Pierre. He learned from them. He was in love with Lisette. Sh e was the contrast to Tashi. She was the opposite of her. Adam probably would have rather lived in France with her, but he stood by Tashi to give her love and affection. Adam was not a bad guy at all. He helped Tashi through the years and was always there whenever she needed somebody. It was unfair of him to be cheating on Tashi with Lisette, and Tashi resented him for that. He was always honest with Tashi though. Adam, like many of the other men in this book, was not a terrible person. He was good at heart.

What Is Cerebral Palsy Health And Social Care Essay

Along with motor upsets, patients can besides hold other medical jobs. Epilepsy, mental deceleration, larning disablements or Attention Deficit Hyperactivity Disorder ( ADHD ) are sometimes seen in patients with intellectual paralysis. Cerebral paralysis is non a progressive upset, intending symptoms will non come on after clip. Congenital intellectual paralysis, intending bing from birth, is the largest figure of instances in the United States. 5 kids in every 2,000 Borns are diagnosed with intellectual paralysis. Today, 5-10 % are diagnosed with athetoid intellectual paralysis, which is characterized by slow, wrestling nonvoluntary motions. The staying 80-90 % are diagnosed with spastic intellectual paralysis. This type is characterized by rigidness in musculuss which causes stiffness and restricted motion in patient. In the 1880 ‘s, intellectual paralysis was believed to be caused by deficiency of O at birth. It has since been found to be caused by encephalon harm in early fetal development, before the procedure of birth. One possibility is an infection in the amnionic fluid environing the foetus. Infections can be rubella ( German rubeolas ) , toxoplasmosis-an infection caused by a one-celled parasite most normally acquired from contact with cats and their fecal matters. These infections are a hazard to the foetus merely if the female parent contracts the infection for the first clip during the gestation. A shot in a foetus can do neurological harm merely like every bit in an grownup. Birth asphyxia important plenty to do intellectual paralysis is uncommon in developed states, although it can still happen. There are infections that can be passed during birth that are n't passed through the placenta during gestation. An infection that causes a serious unwellness in the neonate can hold the possible to bring forth some neurological harm. Approximately 15 % of intellectual paralysis is due to a encephalon hurt sustained after birth. Infections, such as meningitis and phrenitis may do irreversible harm to the encephalon. Abuse, accidents, near drowning, or asphyxiations may do intellectual paralysis in newborns/children up to age 3. After the age of three, the neurological harm is n't classified as intellectual paralysis. A doctor will name intellectual paralysis in a kid by supervising the kid ‘s mileposts, when they sit up, start catching things and when they start walking. Besides observing musculus rigidness or unnatural leg/arm motion in the kid. Children enduring from intellectual paralysis will besides meet other neurological jobs besides the 1s mentioned and besides orthopaedic jobs. Seizures, epilepsy, ADHD, larning disablements, and mental deceleration are a few of the neurological jobs that can travel along with intellectual paralysis. Scoliosis, disagreement in leg length, contractures of articulations, and hip disruption are some of the orthopaedic jobs associated with intellectual paralysis. This is n't to state that every patient diagnosed with intellectual paralysis will see every one of these symptoms. Since it is a neurological upset, no two patients are traveling to be afflicted in the same manner. There are different types of Cerebral paralysis. Spastic is the inability to loosen up a musculus ( stiffness ) and affects approximately 80 % of those with CP. Athetoid is the inability to command a musculus and it affects about 25 % of those with Cerebral Palsy. Hypotonic is where the musculuss are really floppy due to hapless or low musculus tone and may develop spasticity. Hemiplegia is muscle stiffness in one arm and one leg on the same side of the organic structure and affects approximately 1 in every 1,000 kid born. Hemiplegia does n't ever intend your kid has intellectual paralysis. Diplegia is musculuss stiffness in both legs. Quadriplegia is muscle stiffness in all four appendages including the truck and cervix musculuss. This signifier of intellectual paralysis is caused by an hurt to the spinal cord that sends messages to the organic structure parts that control motion and esthesis. Ataxic affects coordination of musculuss, impacting all four limbs and besides the bole. A patient holding atactic intellectual paralysis will hold hapless or low musculus tone. Ataxic intellectual paralysis affects 5-10 % of those with intellectual paralysis. There are different types of therapy that can be used with intellectual paralysis to decrease or command the grade of musculus job. Physical therapy, medicines, orthotic devices and surgery are some types of therapies or interventions that can be used. Physical therapy can assist with musculus preparation, beef uping the musculuss, balance and mobility. Stretching is one manner to assist decelerate the formation of contractures ( joint scope of gesture restrictions ) . Medicines can cut down shudders, musculus cramps and can besides loosen up tight musculuss. Injected medicines can cut down countries of musculus spasticity. Orthotic devices, besides called braces, can be used to stretch spastic musculuss which can forestall loss of abilities and balance. Surgery can be used to rectify size of leg differences and besides to assist rectify any spine curvatures. Cerebral paralysis is a neurological upset caused by infections during gestation, during birth or after birth, physical injury to the caput and/or spinal column, and besides by deficiency of O although that cause has been decreased as medical progresss are made. If affects a big sum of people and can be rather hard to populate with. Since it is a neurological upset, it affects each patient in a different manner. There is no remedy for intellectual paralysis, but there are ways to minimise the effects it can hold on a patient.

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